The Dhamola river pollution is going on increasing day to day. The main source of pollution in river include municipal wastes from Saharanpur urban areas and industrial effluents of sugar, pulp and paper, distilleries and other miscellaneous industries through tributaries as well as direct inputs. The main goal of present study was to assess the impact of urban and industrial activities on the water quality of river Dhamola in Saharanpur and to study physico-chemical analysis of water. The laboratory test of the collected water samples were performed for analysis of various physical and chemical parameters such as pH, Temperature, Turbidity, Color, Total Dissolved Solids (TDS), Total Hardness (TH), Calcium Hardness and Alkalinity. The results obtained from chemical analysis were compared with four standards namely ISI, ICMR, BIS and WHO. It is found that this water body is not suitable for drinking and irrigation purpose, so possible remedial methods should be adopted for this water resource for improving its quality. Keywords: Physico-chemical analysis, Dhamola River, Total Hardness, pH, Alkalinity, Human Impact.